Development and validation of a capillary electrophoresis method for the determination of sulfate in indinavir sulfate raw material
| Autor: | Pereira Elisabete A., Micke Gustavo A., Tavares Marina F. M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: | |
| Zdroj: | Journal of the Brazilian Chemical Society, Vol 17, Iss 2, Pp 251-256 (2006) |
| Druh dokumentu: | article |
| ISSN: | 0103-5053 1678-4790 |
| Popis: | In this work, a simple and fast capillary electrophoresis method using indirect UV detection (230 nm) for the quantitative determination of sulfate in the indinavir sulfate raw material was described. A running electrolyte consisting of 10 mmol L-1 ammonium molybdate containing 0.15 mmol L-1 cetyltrimethylammonium bromide (pH 7.5) was used. Samples were injected hydrodynamically by applying 5000 Pa pressure during 3 s. The response was linear from 10.1 µg mL-1 to 79.8 mg µL-1. Limits of detection and quantification were 0.34 and 1.13 mg µL-1, respectively. The intra-day precision expressed as relative standard deviation was 2.8% for 10 independent sample preparations. Recoveries varying from 96.9 to 102.4% at three concentration levels were obtained. The methodology was successfully applied for real samples. The concentration of sulfate in indinavir sulfate raw material varied from 13.3 to 13.5% (m/m calculated as anhydrous base). |
| Databáze: | Directory of Open Access Journals |
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