Impact of short-term high-fat feeding on lipid droplet content in mouse oocytes
| Autor: | Ryutaro AIZAWA, Megumi IBAYASHI, Tomomi HATAKEYAMA, Takayuki TATSUMI, Satoshi TSUKAMOTO |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | The Journal of Reproduction and Development, Vol 67, Iss 1, Pp 73-77 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0916-8818 1348-4400 37887610 |
| DOI: | 10.1262/jrd.2020-094 |
| Popis: | Mature mammalian oocytes contain lipid droplets (LDs), which are neutral lipid storage organelles critically important for energy metabolism. In mice, maternal obesity, induced by long-term (> 3 months) high-fat feeding, contributes to the accumulation of LDs in mature oocytes. However, few studies have investigated the influence of short-term high-fat feeding on LD content. In this study, we demonstrated that 3 weeks of high-fat feeding is sufficient to increase LD content and intracellular triacylglycerol levels. Using a two-step centrifugation technique to release LDs into the perivitelline space, we found that short-term high-fat feeding increased the level of LDs in MII oocytes and that 3 days of high-fat feeding were sufficient to increase efficiency of LD release. Collectively, our study suggests that short-term high fat feeding can have a higher impact on lipid metabolism during oocyte maturation. |
| Databáze: | Directory of Open Access Journals |
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