Episomal vector-based generation of human induced pluripotent stem cell line MUSIi020-A from peripheral blood T-cells

Autor: Ponthip Pratumkaew, Sudjit Luanpitpong, Phatchanat Klaihmon, Chanchao Lorthongpanich, Chuti Laowtammathron, Sukanya Meesa, Wichuda Suksomboon, Surapol Issaragrisil
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Stem Cell Research, Vol 64, Iss , Pp 102929- (2022)
Druh dokumentu: article
ISSN: 1873-5061
DOI: 10.1016/j.scr.2022.102929
Popis: Human induced pluripotent stem cell (iPSC) line MUSIi020-A was generated from T cells isolated from peripheral blood of a healthy 37-year-old female and reprogrammed using episomal plasmid vectors. The established transgene-free MUSIi020-A, which retained a normal karyotype, displayed pluripotency as characterized by expression of pluripotency markers and by in vitro spontaneous differentiation toward three embryonic germ layers. This cell line may represent a valuable tool for studying T cell development and a potential cell source for cancer immunotherapy.
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