N-terminal amino acid sequences of the major outer membrane proteins from a Neisseria meningitidis group B strain isolated in Brazil
| Autor: | Salvatore Giovanni De Simone, Solange AF Soares, Andre LA Souza, Maria GM Danelli |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 91, Iss 1, Pp 111-116 (1996) |
| Druh dokumentu: | article |
| ISSN: | 0074-0276 1678-8060 |
| DOI: | 10.1590/S0074-02761996000100020 |
| Popis: | The four dominant outer membrane proteins (46, 38, 33 and 28 kDa) were detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) in a semi-purified preparation of vesicle membranes of a Neisseria meningitidis (N44/89, B:4:P1.15:P5.5,7) strain isolated in Brazil. The N-terminal amino acid sequence for the 46 kDa and 28 kDa proteins matched that reported by others for class 1 and 5 proteins respectively, whereas the sequence (25 amino acids) for the 38 kDa (class 3) protein was similar to class 1 meningococcal proteins. The sequence for the 33 kDa (class 4) was unique and not homologous to any known protein. |
| Databáze: | Directory of Open Access Journals |
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