| Autor: |
Jia Cao, Ling Jin, Zi-Qi Yan, Xiao-Kai Wang, You-You Li, Zun Wang, Yi-Wei Liu, Hong-Ming Li, Zhe Guan, Ze-Hui He, Jiang-Shan Gong, Jiang-Hua Liu, Hao Yin, Yi-Juan Tan, Chun-Gu Hong, Shi-Kai Feng, Yan Zhang, Yi-Yi Wang, Lu-Yue Qi, Chun-Yuan Chen, Zheng-Zhao Liu, Zhen-Xing Wang, Hui Xie |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 14, Iss 1, Pp 1-15 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-023-44312-w |
| Popis: |
Abstract Endothelial cells (ECs) and bone marrow stromal cells (BMSCs) play crucial roles in supporting hematopoiesis and hematopoietic regeneration. However, whether ECs are a source of BMSCs remains unclear. Here, we evaluate the contribution of endothelial-to-mesenchymal transition to BMSC generation in postnatal mice. Single-cell RNA sequencing identifies ECs expressing BMSC markers Prrx1 and Lepr; however, this could not be validated using Prrx1-Cre and Lepr-Cre transgenic mice. Additionally, only a minority of BMSCs are marked by EC lineage tracing models using Cdh5-rtTA-tetO-Cre or Tek-CreERT2. Moreover, Cdh5 + BMSCs and Tek + BMSCs show distinct spatial distributions and characteristic mesenchymal markers, suggestive of their origination from different progenitors rather than CDH5+ TEK+ ECs. Furthermore, myeloablation induced by 5-fluorouracil treatment does not increase Cdh5 + BMSCs. Our findings indicate that ECs hardly convert to BMSCs during homeostasis and myeloablation-induced hematopoietic regeneration, highlighting the importance of using appropriate genetic models and conducting careful data interpretation in studies concerning endothelial-to-mesenchymal transition. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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