Age‐related changes in microRNAs expression in cruciate ligaments of wild‐stock house mice

Autor: Yalda A. Kharaz, Katarzyna Goljanek‐Whysall, Gareth Nye, Jane L. Hurst, Anne McArdle, Eithne J. Comerford
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Physiological Reports, Vol 10, Iss 16, Pp n/a-n/a (2022)
Druh dokumentu: article
ISSN: 2051-817X
DOI: 10.14814/phy2.15426
Popis: Abstract Cruciate ligaments (CL) of the knee joint are injured following trauma or aging. MicroRNAs (miRs) are potential therapeutic targets in musculoskeletal disorders, but there is little known about the role of miRs and their expression ligaments during aging. This study aimed to (1) identify if mice with normal physical activity, wild‐stock house mice are an appropriate model to study age‐related changes in the knee joint and (2) investigate the expression of miRs in aging murine cruciate ligaments. Knee joints were collected from 6 and 24 months old C57BL/6 and wild‐stock house mice (Mus musculus domesticus) for ligament and cartilage (OARSI) histological analysis. Expression of miR targets in CLs was determined in 6‐, 12‐, 24‐, and 30‐month‐old wild‐stock house mice, followed by the analysis of predicted mRNA target genes and Ingenuity Pathway Analysis. Higher CL and knee OARSI histological scores were found in 24‐month‐old wild‐stock house mice compared with 6‐ and 24‐month‐old C57BL/6 and 6‐month‐old wild‐stock house mice (p
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