| Autor: |
Yi-qing He, Wen-wen Liao, Mi Tao, Yu-yan Li, Ping Gao |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Linchuang shenzangbing zazhi, Vol 24, Iss 5, Pp 399-405 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1671-2390 |
| DOI: |
10.3969/j.issn.1671-2390.2024.05.008 |
| Popis: |
Objective To explore the effect of active vitamin D on renin, (pro) renin receptor (PRR) and NF-κB in rat renal tubular epithelial (NRK-52E) cells in high glucose conditions and elucidate the role of NF-κB. Methods NRK-52E cells were cultured in vitro and divided into 7 groups of control, high glucose, high glucose + PDTC (pyrrolidinedithiocarbamate ammonium) and high glucose + 0.1/1/10/100 nM paricalcitol. mRNA and protein expression of renin, PRR and NF-κB p65 in each group were detected by quantitative polymerase chain reaction (qPCR) and Western blot. ChIP-qPCR was utilized for detecting the relationship between NF-κB p65 and the promoter of renin. Results The expressions of renin, PRR and NF-κB spiked in high-glucose group as compared with control group (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
