EMS-induced mutagenesis in Choy sum (Brassica chinensis var. parachinensis) and selection for low light tolerance using abiotic stress indices

Autor: Subramanian Deivanai, Benny Jian Rong Sng, Kien Van Vu, Thankaraj Salammal Maria Shibu, In-Cheol Jang, Srinivasan Ramachandran
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: BMC Plant Biology, Vol 23, Iss 1, Pp 1-20 (2023)
Druh dokumentu: article
ISSN: 1471-2229
DOI: 10.1186/s12870-023-04570-8
Popis: Abstract Background Choy Sum (Brassica rapa ssp. chinensis var. parachinensis), grown in a controlled environment, is vulnerable to changes in indoor light quality and displays distinct photo-morphogenesis responses. The scarcity of Choy Sum germplasm for indoor cultivation necessitates the development of new cultivars. Hence, this study attempted to develop mutants through chemical mutagenesis and select low-light-tolerant mutants by using abiotic stress tolerance indices. Results A mutant population of Choy Sum created using 1.5% ethyl methane sulfonate (EMS) at 4 h was manually pollinated to obtain the M2 generation. 154 mutants with reduced hypocotyl length were initially isolated from 3600 M2 seedlings screened under low light (R: FR = 0.5). Five mutants that showed reduced plant height at mature stages were selected and screened directly for shade tolerance in the M3 generation. Principal component analysis based on phenotypic data distinguished the M3 mutants from the wild type. Abiotic stress tolerance indices such as relative stress index (RSI), stress tolerance index (STI), geometric mean productivity (GMP), yield stability index (YSI), and stress resistance index (SRI) showed significant (P
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