| Autor: |
Thomas G. Francis, Oihane Jaka, Georgina M. Ellison‐Hughes, Norman R. Lazarus, Stephen D.R. Harridge |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
JCSM Rapid Communications, Vol 5, Iss 2, Pp 226-238 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2617-1619 |
| DOI: |
10.1002/rco2.67 |
| Popis: |
Abstract Background The age‐related loss of muscle mass and quality, sarcopenia, has many contributing factors, one of which may be cellular senescence, but this is not well defined in human skeletal muscle. Method Primary cells were isolated from biopsy samples of the vastus lateralis muscle from healthy adult males (n = 6, 22 ± 1 years), sorted (magnetic activated cell sorting) and chemically induced (doxorubicin, DOX, 0.2 μM) to a senescent state. This allowed the parallel and simultaneous investigation of the two main skeletal muscle‐derived cell types: satellite cell‐derived CD56+ve/desmin+ve myoblasts (muscle precursor cells) and CD56− ve/TE7+ve fibroblasts (at >95% purity). Both cell types were followed for up to 35 days post DOX treatment with a combination of quantitative immunocytochemistry and qRT‐PCR for senescent markers and senescence‐associated secretory phenotype (SASP) factors. Results Myoblasts and fibroblasts showed temporal and quantitative differences in many of the senescence markers studied. p16 protein expression increased across the time course (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text