| Autor: |
Dereje Leta, Gadissa Gutema, Gebremedhin Gebremichael Hagos, Regasa Diriba, Gutema Bulti, Tolawak Sura, Desta Ayana, Dawit Chala, Boki Lenjiso, Jaleta Bulti, Saro Abdella, Habteyes Hailu Tola |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
BMC Research Notes, Vol 15, Iss 1, Pp 1-6 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1756-0500 |
| DOI: |
10.1186/s13104-022-06184-z |
| Popis: |
Abstract Objective This study aimed to investigate the effect of heat inactivation and chemical bulklysis on SARS-CoV-2 detection. Results About 6.2% (5/80) of samples were changed to negative results in heat inactivation at 60 °C and about 8.7% (7/80) of samples were changed to negative in heat inactivation at 100 °C. The Ct values of heat-inactivated samples (at 60 °C, at 100 °C, and bulk lysis) were significantly different from the temperature at 56 °C. The effect of heat on Ct value should be considered when interpreting diagnostic PCR results from clinical samples which could have an initial low virus concentration. The efficacy of heat-inactivation varies greatly depending on temperature and duration. Local validation of heat-inactivation and its effects is therefore essential for molecular testing. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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