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Objective To investigate the infectious status, virulence gene, molecular typing and antibiotic resistance of diarrheogenic Escherichia coli (DEC) in sentinel hospitals of Fujian Province in 2019. Methods Fluorescent polymerase chain reaction (PCR) was used to identify 210 fecal samples of diarrhea patients after isolation of E. coli according to GB 4789. 6-2016, and then pulsed field gel electrophoresis (PFGE) molecular traceability and antibiotic resistance test were conducted on the isolated DEC. Results Thirty two strains of bacteria were detected, with a detection rate of 15.2% (32/210). Among them, enteropathogenic E. coli (EPEC) accounted for 37.5% (12/32), enteroaggregative E. coli (EAEC) for 37.5% (12/32), and enterotoxigenic E. coli (ETEC) for 25.0% (8/32). The results of antibiotic resistance test showed that these 32 strains of bacteria were most resistant to ampicillin, with a resistance rate of 78.1% (25/32), followed by tetracycline and trimethoprim/sulfamethoxazole, with resistance rates of 62.5% (20/32) and 59.4% (19/32), respectively. The multiple antibiotic resistance rate was 50.0% (16/32). The results of PFGE showed that 32 strains of bacteria causing diarrhea were divided into 28 PFGE banding patterns. Among them, 12 strains of EPEC and 12 strains of EAEC were divided into 10 PFGE banding patterns, respectively, and 8 strains of ETEC were divided into 8 PFGE banding patterns. The results of cluster analysis showed that two groups of EPEC strains had 100.0% similar banding patterns, one group of EAEC strains had 100.0% similar banding patterns, and ETEC strains did not have completely consistent banding pattern. Conclusion EPEC and EAEC were the main pathogens of diarrhea in surveillance points of Fujian Province in 2019. The genetic diversity of the strains showed that the genetic relationship between them was relatively distant. The antibiotic resistance of DEC was severe, and the rate of multiple antibiotic resistance was high. |
