Porcine hemothropic mycoplasmas infection associated with productive impact in intensive pig production

Autor: Fernando Antônio Moreira Petri, Karina Sonalio, Henrique Meiroz de Souza Almeida, Maria Eugênia Silveira Ferraz, Gabriel Yuri Storino, Mauro Rodrigo de Souza, Marcos Rogério André, Luis Guilherme de Oliveira
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Porcine Health Management, Vol 6, Iss 1, Pp 1-8 (2020)
Druh dokumentu: article
ISSN: 2055-5660
DOI: 10.1186/s40813-020-00171-1
Popis: Abstract Background So far, three porcine hemoplasmas (PH) have been identified, namely Mycoplasma suis, Mycoplasma parvum, and Mycoplasma haemosuis. The first one is the main agent associated with porcine hemoplasmosis, a possible cause of economic losses in pig production. Thus, this work aimed to detect and quantify PH 16S rRNA in finishing pigs and to associate its load estimate with average daily weight gain (ADWG). For this purpose, whole blood samples from 318 pigs were collected at an age of 75 days (d0) when the pigs entered the finishing phase and 105 days later (d105). To calculate ADWG, the animals were weighed at the abovementioned dates. Then, DNA from blood samples were submitted to a qPCR targeting the 16S rRNA gene for PH. Spearman correlation test was performed to investigate potential associations between ADWG and the quantification values. Lastly, the molecular characterization of PH was done by sequencing the 23S rDNA gene. Results Out of the 318 samples, 190 (59.74%) were positive on d0, and 304 (95.6%) were positive on d105. A significant correlation was observed (p
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