Autor: |
Camila E Minuzzi, Luiza Pires Portella, Patricia Bräunig, Luis Antonio Sangioni, Aline Ludwig, Luciane Silva Ramos, Liliane Pacheco, Camila Ribeiro Silva, Flávia Caselli Pacheco, Ivone Andreatta Menegolla, Lourdes Bonfleur Farinha, Priscila Pauli Kist, Regina Mitsuka Breganó, Beatriz de Souza Lima Nino, Felippe Danyel Cardoso Martins, Thais Cabral Monica, Fernanda Pinto Ferreira, Isadora Britto, Ariana Signori, Kerlei Cristina Medici, Roberta Lemos Freire, João Luis Garcia, Italmar Teodorico Navarro, Cledison Marcio Difante, Fernanda Silveira Flores Vogel |
Jazyk: |
angličtina |
Rok vydání: |
2020 |
Předmět: |
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Zdroj: |
PLoS ONE, Vol 15, Iss 1, p e0228442 (2020) |
Druh dokumentu: |
article |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal.pone.0228442 |
Popis: |
Toxoplasma gondii is a protozoan that has great genetic diversity and is prevalent worldwide. In 2018, an outbreak of toxoplasmosis occurred in Santa Maria, Brazil, which was considered the largest outbreak ever described in the world. This paper describes the isolation and molecular characterization of Toxoplasma gondii from the placenta of two pregnant women with acute toxoplasmosis who had live births and were receiving treatment for toxoplasmosis during the outbreak. For this, placental tissue samples from two patients underwent isolation by mice bioassay, conventional PCR and genotyping using PCR-RFLP with twelve markers. Both samples were positive in isolation in mice. The isolate was lethal to mice, suggesting high virulence. In addition, the samples were positive in conventional PCR and isolates submitted to PCR-RFLP genotyping presented an atypical genotype, which had never been described before. This research contributes to the elucidation of this great outbreak in Brazil. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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