A simple, rapid and economic method for detecting multidrug-resistant tuberculosis
| Autor: | Xia Wang, Junhua Jiao, Weihua Xu, Xiaoyan Chai, Zhenyun Li, Qingjiang Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Infectious Diseases, Vol 17, Iss 6, Pp 667-671 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1413-8670 55465161 |
| DOI: | 10.1016/j.bjid.2013.04.008 |
| Popis: | Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 isolates were sensitive to isonicotinylhydrazine and rifampin. Primers were designed to target five mutations hotspots that confer resistance to the first-line drugs isoniazid and rifampin, and multiplex allele specific polymerase chain reaction was performed. Whole-genome sequencing confirmed drug resistance mutations identified by multiplex allele specific polymerase chain reaction. Results: DNA sequencing revealed that 68.9% of multidrug-resistant strains have point mutations at codon 315 of the katG gene, 19.8% within the mabA-inhA promoter, and 98.0% at three hotspots within rpoB. Multiplex allele specific polymerase chain reaction detected each of these five mutations, yielding 82.3% sensitivity and 100% specificity for isoniazid resistance, and 97.9% sensitivity and 100% specificity for rifampin resistance as compared to drug susceptibility testing. Conclusions: The results show that multiplex allele specific polymerase chain reaction is an inexpensive and practical method for rapid detection of multidrug-resistant tuberculosis in developing countries. Keywords: Mycobacterium tuberculosis, Multiplex allele specific polymerase chain reaction (MAS-PCR) |
| Databáze: | Directory of Open Access Journals |
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