Transplanted Peripheral Blood Stem Cells Mobilized by Granulocyte Colony-Stimulating Factor Promoted Hindlimb Functional Recovery after Spinal Cord Injury in Mice

Autor: Hiroshi Takahashi, Masao Koda M.D., Ph.D., Masayuki Hashimoto, Takeo Furuya, Tsuyoshi Sakuma, Kei Kato, Akihiko Okawa, Taigo Inada, Koshiro Kamiya, Mitsutoshi Ota, Satoshi Maki, Kazuhisa Takahashi, Masashi Yamazaki, Chikato Mannoji
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Cell Transplantation, Vol 25 (2016)
Druh dokumentu: article
ISSN: 0963-6897
1555-3892
DOI: 10.3727/096368915X688146
Popis: Granulocyte colony-stimulating factor (G-CSF) mobilizes peripheral blood stem cells (PBSCs) derived from bone marrow. We hypothesized that intraspinal transplantation of PBSCs mobilized by G-CSF could promote functional recovery after spinal cord injury. Spinal cords of adult nonobese diabetes/severe immunodeficiency mice were injured using an Infinite Horizon impactor (60 kdyn). One week after the injury, 3.0 μl of G-CSF-mobilized human mononuclear cells (MNCs; 0.5 × 10 5 /μl), G-CSF-mobilized human CD34-positive PBSCs (CD34; 0.5 × 10 5 /μl), or normal saline was injected to the lesion epicenter. We performed immunohistochemistry. Locomotor recovery was assessed by Basso Mouse Scale. The number of transplanted human cells decreased according to the time course. The CD31-positive area was significantly larger in the MNC and CD34 groups compared with the vehicle group. The number of serotonin-positive fibers was significantly larger in the MNC and CD34 groups than in the vehicle group. Immunohistochemistry revealed that the number of apoptotic oligodendrocytes was significantly smaller in cell-transplanted groups, and the areas of demyelination in the MNC- and CD34-transplanted mice were smaller than that in the vehicle group, indicating that cell transplantation suppressed oligodendrocyte apoptosis and demyelination. Both the MNC and CD34 groups showed significantly better hindlimb functional recovery compared with the vehicle group. There was no significant difference between the two types of transplanted cells. Intraspinal transplantation of G-CSF-mobilized MNCs or CD34-positive cells promoted angiogenesis, serotonergic fiber regeneration/sparing, and preservation of myelin, resulting in improved hindlimb function after spinal cord injury in comparison with vehicle-treated control mice. Transplantation of G-CSF-mobilized PBSCs has advantages for treatment of spinal cord injury in the ethical and immunological viewpoints, although further exploration is needed to move forward to clinical application.
Databáze: Directory of Open Access Journals
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