Evaluation of chemokines in gingival crevicular fluid in children with dental caries and stainless steel crowns: A clinico-biochemical study
| Autor: | Naveen Kommineni Kumar, Veera Kishore Kasa Reddy, Prathyusha Padakandla, Harshini Togaru, Swathi Kalagatla, Sarath N Chandra |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Indian Society of Pedodontics and Preventive Dentistry, Vol 34, Iss 3, Pp 273-279 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0970-4388 1998-3905 |
| DOI: | 10.4103/0970-4388.186754 |
| Popis: | Aims and Objectives: The study was conducted to detect the presence of macrophage inflammatory protein-1α (MIP-1α) and MIP-1β and estimate their levels in gingival crevicular fluid (GCF) in children with dental caries and stainless steel crowns. Materials and Methods: A total of 80 children with primary dentition were selected and categorized into four groups with twenty in each group; Group 1 - healthy subjects, Group 2 - dental caries, Group 3 - dental caries involving the pulp, and Group 4 - stainless steel crowns. GCF samples were collected by an extra-crevicular method with microcapillary pipettes. The GCF samples were quantified by ELISA and the levels of MIP-1α and MIP-1β were determined. Results: MIP-1α and MIP-1β were detected in all the samples. Highest mean concentration in GCF was obtained for Group 3 followed by Groups 2 and 4 while the lowest concentration was seen in Group 1. This suggests that MIP-1α and MIP-1β levels in GCF increased proportionately with the inflammation. Conclusions: GCF serves as a noninvasive diagnostic fluid to measure biomarkers released during dental caries initiation and progression. MIP-1α and MIP-1β chemokines can be considered as novel biomarkers, in biological mechanism underlying the pathogenesis and inflammation in children with dental caries and stainless steel crowns. |
| Databáze: | Directory of Open Access Journals |
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