| Autor: |
Hyun Young Kim, Wonseok Lee, Xiao Liu, Haeum Jang, Sadatsugu Sakane, Raquel Carvalho-Gontijo Weber, Karin Diggle, Samuel A. Kerk, Christian M. Metallo, Tatiana Kisseleva, David A. Brenner |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 5, Iss 2, Pp 103111- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2024.103111 |
| Popis: |
Summary: Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce metabolic and alcohol-associated hepatic steatosis, inflammation, and fibrosis. We outline potential applications, including using human liver spheroids for experimental and translational research and drug screening to identify potential anti-fibrotic therapies. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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