| Autor: |
Adauto Tsutomu Ikejiri, Frederico Somaio Neto, Paulo Roberto Bertoletto, José Carlos Chaves, Alexandre Key WakateTeruya, Cândida Aparecida Leite Kassuya, Murched Omar Taha, Djalma José Fagundes |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Acta Cirúrgica Brasileira, Vol 33, Iss 5, Pp 462-471 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
0102-8650 |
| DOI: |
10.1590/s0102-865020180050000009 |
| Popis: |
Abstract Purpose: To evaluate the effect of hyperbaric oxygenation (HBO) on the expression of the genes antioxidant glutathione peroxidase 4 (Gpx4) and lactoperoxidase (Lpo) in the lung of mice subjected to intestinal ischemia and reperfusion (IIR). Methods: Control group (CG) in which were subjected to anesthesia, laparotomy and observation for 120 minutes; an ischemia and reperfusion group (IRG) subjected to anesthesia, laparotomy, small bowel ischemia for 60 minutes and reperfusion for 60 minutes; and three groups treated with HBO during ischemia (HBOG + I), during reperfusion (HBOG + R) and during ischemia and reperfusion (HBOG + IR). Studied 84 genes of oxidative stress by the method (RT-qPCR). Genes with expression levels three times below or above the threshold cycle were considered significantly hypoexpressed or hyperexpressed, respectively (Student’s t-test p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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