The effect of different sweeteners on the oral microbiome: a randomized clinical exploratory pilot study

Autor: Davis R. Zakis, Bernd W. Brandt, Suzette V. van der Waal, Bart J. F. Keijser, Wim Crielaard, Derek W.K. van der Plas, Catherine M.C. Volgenant, Egija Zaura
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Oral Microbiology, Vol 16, Iss 1 (2024)
Druh dokumentu: article
ISSN: 20002297
2000-2297
DOI: 10.1080/20002297.2024.2369350
Popis: Introduction The aim of the study was to evaluate the modulating effects of five commonly used sweetener (glucose, inulin, isomaltulose, tagatose, trehalose) containing mouth rinses on the oral microbiome.Methods A single-centre, double-blind, parallel randomized clinical trial was performed with healthy, 18–55-year-old volunteers (N = 65), who rinsed thrice-daily for two weeks with a 10% solution of one of the allocated sweeteners. Microbiota composition of supragingival dental plaque and the tongue dorsum coating was analysed by 16S RNA gene amplicon sequencing of the V4 hypervariable region (Illumina MiSeq). As secondary outcomes, dental plaque red fluorescence and salivary pH were measured.Results Dental plaque microbiota changed significantly for two groups: inulin (F = 2.0239, p = 0.0006 PERMANOVA, Aitchison distance) and isomaltulose (F = 0.67, p = 0.0305). For the tongue microbiota, significant changes were observed for isomaltulose (F = 0.8382, p = 0.0452) and trehalose (F = 1.0119, p = 0.0098). In plaque, 13 species changed significantly for the inulin group, while for tongue coating, three species changed for the trehalose group (ALDEx2, p
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