In vivo regeneration of neo-nephrons in rodents by renal progenitor cell transplantation

Autor: Shuichiro Yamanaka, Kenji Matsui, Toshinari Fujimoto, Tsuyoshi Takamura, Yatsumu Saito, Naoto Matsumoto, Susumu Tajiri, Kei Matsumoto, Takashi Yokoo
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 1, Pp 100314- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100314
Popis: Summary: Renal progenitor cells induced from pluripotent stem cells have attracted attention as a cell source for organ regeneration. Here, we report an in vivo protocol for the regeneration of urine-producing nephrons, i.e., neo-nephrons, in mice. We outline steps to transplant exogenous renal progenitor cells into the nephrogenic zone of transgenic mice and subsequently analyze these neo-nephrons.For complete details on the use and execution of this protocol, please refer to Fujimoto et al. (2020).
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