Pengaruh Hormon Androgen terhadap Ekspresi Gen CD52 di Epididimis Mencit (Mus musculus)
| Autor: | Silvani Permatasari, Dwi Ari Pujianto, Astrid Teresa |
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| Jazyk: | English<br />Indonesian |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Jurnal Kesehatan Andalas, Vol 9, Iss 2, Pp 182-188 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2301-7406 2615-1138 |
| DOI: | 10.25077/jka.v9i2.1224 |
| Popis: | Androgen has an important role in regulation epididymal sperm maturation Epididymal sperm maturation is occurs via interactions between sperm and proteins secreted by epididymal genes that are specifically expressed in a region-specific manner. Mice CD52 exhibited expression in all regions in epididymis. However, the regulation of its expression by androgen is needed to be known as a gene candidate that involved in sperm maturation. Objective: This study was aimed to analyze regulation of mice CD52 expression by androgen and a bioinformatics analysis was performed to predict functional domains of CD52. Methods: bioinformatics analysis using Motif Scan. For experiment, mice divided into 5 groups. The groups were control was injected ethanol 0,1 mL/day and others groups were 3, 5, 10, and 15 day used a androgen receptor antagonist, flutamide that dilute in ethanol at a dose of 50 mg/kg/day. And then quantitative real-time RT-PCR was used to analyse expression of CD52 and it’s normalized to Beta actin. Result: CD52 contained a two potential phosphorylation sites for protein kinase C and casein kinase II, N-myristoylation, and N-glycosylation that showed CD52 is secretory protein and predict it’s attached to sperm membrane. Expression of CD52 was decrease about 93% than control. Conclusion: CD52 expression depend on androgen. Keywords: androgen; epididymis; sperm maturation; expression of CD52 |
| Databáze: | Directory of Open Access Journals |
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