| Autor: |
Wasin Somboot, Suphakit Awiphan, Jaroon Jakmunee, Tippawan Prapamontol, Tinakorn Kanyanee |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Talanta Open, Vol 7, Iss , Pp 100208- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-8319 |
| DOI: |
10.1016/j.talo.2023.100208 |
| Popis: |
Measurement of ammonia in exhaled breath is of interest in medical and health sciences. The fluorometric determination of NH3 in exhaled breath condensate (EBC) was developed based on a digital image of a windowless falling drop (WFD) cell at the end of tube via a sensitive and selective reaction of NH3-OPA-sulfite in flow injection analysis system (FIA). A low-cost USB microscope as a facile instrumentation without expensive optical parts, an amplifier circuit, and a data acquisition unit, was used as a fluorescence light detector for the WFD cell via instant reporting temporal profile of digital value. A linear calibration graph of NH4+ in the range of 0.5–10 µmol L−1 can be obtained with a limit of detection of 0.5 µmol L−1 NH4+, and repeatability of 5.5% RSD (n = 11). The fluorescence (FL) signal obtained from the low-cost USB microscope was not different from that using the photodiode detector via a cross-shape flow cell. The proposed device was successfully applied for NH4+ determination in EBC and air absorbing solution samples with the recovery of 98–110%. The approach for instant reporting of a temporal profile of digital image value from a camera shows a potential to be applied for rapid chemical analysis in other microfluidics systems. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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