Suppressive effects of the neutrophil elastase inhibitor sivelestat sodium hydrate on interleukin-1β production in lipopolysaccharide-stimulated porcine whole blood
| Autor: | Yasuyoshi Kurimoto, Yasuyo Shimomura, Kazuhiro Moriyama, Tomoyuki Nakamura, Naohide Kuriyama, Yoshitaka Hara, Hidefumi Komura, Daisuke Hasegawa, Takahiro Kawaji, Osamu Nishida |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Fujita Medical Journal, Vol 6, Iss 1, Pp 12-16 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2189-7247 2189-7255 |
| DOI: | 10.20407/fmj.2019-002 |
| Popis: | Objective: Sivelestat sodium hydrate (Siv) is expected to be an effective therapy for acute respiratory distress syndrome, although its mechanism of action is not understood. In this study, we investigated which myeloid cells-derived cytokines were suppressed by Siv. Methods: Continuous hemofiltration was performed by circulating fresh porcine blood through a semi-closed circuit. To ensure that leukocytes survived for 360 min, 5% glucose, heparin, and air were continuously injected. The control group received continuous administration of lipopolysaccharide (LPS) only, whereas the Siv group received LPS and Siv. Complete blood count, levels of various cytokines, and other variables were compared between the groups. Results: Interleukin (IL)-1β level was significantly suppressed in the Siv group compared with that in the control group (p |
| Databáze: | Directory of Open Access Journals |
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