Treatment with conditioned culture media with human mesenchymal stem cells on reserpine-induced thermal fibromyalgia model in rats
| Autor: | Farzaneh Jasbinia, Majid Hassanpourezatti |
|---|---|
| Jazyk: | perština |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | مجله دانشگاه علوم پزشکی بیرجند, Vol 29, Iss 3, Pp 218-228 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1607-2197 2423-6152 |
| Popis: | Background and Aims: Fibromyalgia (FM) is a chronic pain syndrome affected by the hippocampal function. Mesenchymal stem cells-conditioned medium (MSC-CMs) has shown antioxidant and neuroprotective potentials in treating various diseases. The present study aimed to investigate the efficacy of MSC-CMs on the thermal treatment of reserpine-induced FM and evaluate the concentration changes of tumor necrosis factor-alpha (TNF-α) in the hippocampus of rats under these conditions. Materials and Methods: 220 g. Animals received reserpine (1 mg/kg) in the FM groups, and subcutaneous injection of normal saline in other groups, once a day for 3 consecutive days. Then, the rats received MSC-CM (0.3 and 0.5 mg/ml protein content, intraperitoneally) once a week for three weeks. The thermal pain threshold of mice was evaluated using tail flick and hot plate tests at the beginning and end of the experiments. TNF-α concentration in the hippocampus was determined by an ELISA kit at the end of the experimental period. Results: The thermal pain threshold of rats in the FM group decreased significantly (P |
| Databáze: | Directory of Open Access Journals |
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