Contamination Status of Psychrotolerant Morganella psychrotolerans in Fish and Histamine-Producing Capacity of Its Isolates

Autor: LI Jin, WANG Di, CHEN Shengjun, WU Yanyan, LI Chunsheng, WANG Yueqi
Jazyk: English<br />Chinese
Rok vydání: 2024
Předmět:
Zdroj: Shipin Kexue, Vol 45, Iss 5, Pp 275-282 (2024)
Druh dokumentu: article
ISSN: 1002-6630
DOI: 10.7506/spkx1002-6630-20230830-222
Popis: The contamination status of cold resistant Morganella psychrotolerans in commercially available aquatic products was investigated, and the generation of histamine by its isolates was explored. A total of 100 samples of commercially available fish from Guangdong province were surveyed. The results revealed a widespread distribution of M. psychrotolerans with a contamination rate of 34%. The contamination rates for red-fleshed and white-fleshed fish were 35.9% and 27.3%, respectively. Specifically, the mackerel samples had a contamination rate of 38.24%, and the tuna samples had a contamination rate of 29.41% among all positive samples. Sixteen strains of M. psychrotolerans were isolated from the positive samples, and all isolates produced histamine levels exceeding 1 000 mg/L after 48 h incubation at 20 ℃. Notably, isolate 1 showed higher histamine-producing capacity than the type strain. Although the two strains exhibited no significant differences in growth curves at varying temperatures (4 and 20 ℃), their histamine-producing capacities were different. After 10 days of culture at 4 ℃, histamine production was higher by isolate 1 than the type strain, while the opposite result was observed after 60 h of culture at 20 ℃. These findings offer crucial theoretical support for addressing the problem of histamine accumulation in cold chain aquatic products.
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