microRNA-214-mediated UBC9 expression in glioma
| Autor: | Zhiqiang Zhao, Xiaochao Tan, Ani Zhao, Liyuan Zhu, Bin Yin, Jiangang Yuan, Boqin Qiang & Xiaozhong Peng |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | BMB Reports, Vol 45, Iss 11, Pp 641-646 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1976-6696 1976-670X |
| Popis: | It has been reported that ubiquitin-conjugating enzyme 9 (Ubc9),the unique enzyme2 in the sumoylation pathway, is up-regulatedin many cancers. However, the expression and regulation ofUBC9 in glioma remains unknown. In this study, we found thatUbc9 was up-regulated in glioma tissues and cell lines comparedto a normal control. UBC9 knockdown by small interfering RNA(siRNA) affected cell proliferation and apoptosis in T98G cells.Further experiments revealed that microRNA (miR)-214 directlytargeted the 3' untranslated region (UTR) of UBC9 and that therewas an inverse relationship between the expression levels ofmiR-214 and UBC9 protein in glioma tissues and cells. miR-214overexpression suppressed the endogenous UBC9 protein andaffected T98G cell proliferation. These findings suggest thatmiR-214 reduction facilitates UBC9 expression and is involvedin the regulation of glioma cell proliferation |
| Databáze: | Directory of Open Access Journals |
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