In-vitro generation of follicle-like structures from human germ cell-like cells derived from theca stem cell combined with ovarian somatic cells

Autor: Seyedeh Nasim Mirbahari, Christiani A. Amorim, Fatemeh Hassani, Mehdi Totonchi, Mahnaz Haddadi, Mojtaba Rezazadeh valojerdi, Azam Dalman
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Ovarian Research, Vol 17, Iss 1, Pp 1-11 (2024)
Druh dokumentu: article
ISSN: 1757-2215
DOI: 10.1186/s13048-023-01315-x
Popis: Abstract Background The objective of this study was to induce the differentiation of human theca stem cells (hTSCs) into germ cell-like cells (hGCLCs) and assess their developmental progression following in vitro 3D culture with ovarian somatic cells within the follicle-like structures. To achieve this, the hTSCs were isolated from small antral follicles of three patients of varying ages and were then seeded in a differentiation medium for 40 days. The differentiated hGCLCs were subsequently aggregated with somatic ovarian cells (cumulus cells and hTSCs) in a ratio of 1:10 and cultured in a growth medium in a suspension culture dish. In addition to examining the morphologies, sizes, and viabilities of the differentiated hGCLCs, this study also analyzed the expression of DAZL and GDF9 proteins within the follicle-like structures. Results After 12 days, the hTSCs began to differentiate into hGCLCs, with their shapes changing from spindle-shaped to spherical. The sizes of hGCLCs increased during the differentiation period (from 25 μm to 50 μm). The survival rate of the hGCLCs after differentiation and in vitro development in primordial follicle-like structures was 54%. Unlike hTSCs, which did not express the DAZL protein, the hGCLCs and follicle-like structures successfully expressed DAZL protein (P-value
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