| Autor: |
Jorge Otero, Miguel A. Rodríguez-Lázaro, Arturo Martínez-Trejo, Daniel Mbanze, Gorka Solana, Andrea Vergara, Salvador Bosch, David Gozal, Jordi Vila, Ramon Farré |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
HardwareX, Vol 19, Iss , Pp e00568- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2468-0672 |
| DOI: |
10.1016/j.ohx.2024.e00568 |
| Popis: |
Loop-Mediated Isothermal Amplification (LAMP) is a useful technique for detecting infectious microorganisms in human fluids since it performs similarly to conventional PCR, the results are obtained faster and no thermocyclers or complex devices are required. Since only two isothermal blocks (95 °C to lyse cells and 65 °C for DNA amplification) are needed, LAMP is particularly suited for applications in Low- and Middle-Income Countries (LMICs). To validate such assumption, we first designed and tested Arduino-controlled LAMP thermoblocks to process a considerable number of samples simultaneously with a low-energy consumption to enable routine use under worst-case conditions (no main power source and low ambient temperatures). The thermoblocks were tested when battery-powered at temperature down to 5 °C, showing high stability in well temperatures ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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