Expression and Diagnostic Value of NPTX1 in Thymoma Patients
| Autor: | Xin DU, Lei YU, Ling YANG, Dingfang CAO, Ying ZHANG |
|---|---|
| Jazyk: | čínština |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Chinese Journal of Lung Cancer, Vol 24, Iss 1, Pp 1-6 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1009-3419 1999-6187 |
| DOI: | 10.3779/j.issn.1009-3419.2021.102.03 |
| Popis: | Background and objective Thymomas are the most common primary malignant tumors of anterior mediastinal. However, there are no specific laboratory indicator for the diagnosis the diagnosis of thymoma. The aim of this study was to screen out a tumor marker for diagnosis of thymoma by mRNA microarray analysis and confirmed it. Methods By mRNA microarray analysis of 31 thymomas and peritumoral thymic tissues, we found that the transcription level of neuronal pentraxin 1 (NPTX1) gene was up-regulated more than 4 times in thymomas. To further verify the above results, we detected the transcription and expression level of NPTX1 in 60 thymoma and 30 thymic cyst patients by quantitative Real-Time polymerase chain reaction (PCR), immunohistochemistry and enzyme-linked immunosorbent assay (ELISA). Furthermore, the diagnostic value of NPTX1 in thymoma by receiver operating characteristic curve (ROC) was analyzed. Results The transcription level of NPTX1 mRNA in thymoma tissues was significantly higher than that in the thymic tissues of control group [(2.88±1.02) vs (1.35±0.47), P |
| Databáze: | Directory of Open Access Journals |
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