Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis
| Autor: | Milanjeet, Harkirat Singh, N.K. Singh, N.D. Singh, Chanchal Singh, S.S. Rath |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Veterinární Medicína, Vol 59, Iss 3, Pp 129-136 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0375-8427 1805-9392 |
| DOI: | 10.17221/7380-VETMED |
| Popis: | Evaluation of blood samples collected from 214 dogs from Ludhiana, Punjab (India) was performed for the presence of Ehrlichia canis using PCR-based assays targeting a portion of the 16S rRNA gene. Of the total samples subjected to routine blood smear examination, the morulae of E. canis were detected in 2.34% samples. Nested PCR assay produced amplicons of expected size (389 bp) specific for E. canis in 41.59% (89/214) of samples. The results of multivariate analysis showed that the prevalence of E. canis was higher in the summer as compared to the winter season (P = 0.031) and in dogs younger than six-month-old as compared to older dogs (P < 0.001), while breed and sex of the host were not significantly associated with the occurrence of the disease. |
| Databáze: | Directory of Open Access Journals |
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