SPREADING AND GENOTYPIC STRUCTURE OF ROTAVIRUSES IN THE ODESA REGION

Autor: В. О. Гончаров, Л. С. Котлік, Н. П. Ісакова, Ю. М. Виходцева, Ю. О. Баранов
Jazyk: English<br />Russian<br />Ukrainian
Rok vydání: 2016
Předmět:
Zdroj: Mìkrobìologìâ ì Bìotehnologìâ, Vol 0, Iss 1(33), Pp 47-54 (2016)
Druh dokumentu: article
ISSN: 2076-0558
2307-4663
DOI: 10.18524/2307-4663.2016.1(33).65366
Popis: Aim. The study was to determine the distribution and genotypic diversity of rotaviruses in children under the age of 5 years, the circulation of rotavirus and other viral pathogens of intestinal infections in tap water and in the environment (waste water) in the Odesa region in 2010-2015. Methods. Determination of rotavirus antigen was determined by enzyme immunoassay, and the determination of nucleic acid of rotaviruses, astroviruses, caliciviruses, enteroviruses, adenoviruses by polymerase chain reaction with reverse transcription (RT-PCR). Results. During the study period, the percentage of allocation of rotavirus sick children at acute intestinal infection was 41.8%. The greatest number of positive samples among children aged 2–5 years – 52%. Based on these data the incidence of rotavirus is recorded all year round. There is a peculiarity in virus circulation. There were registered two uncharacteristic summer peaks of infection in July and August (2012–2015). The prevailing was propagation among genotypes of rotavirus G4P(8), G1P(8), G3P(8) and G2P(4). The examination of faeces and waste water samples revealed that rotaviruses, among other activators of intestinal infections, were found out in a significant number (37.2%). Conclusions. Thus, it was found that among the pathogens of acute enteritis at infected children and in the waste water there were dominated genotypes G4P(8), G1P(8), G3P(8) and G2P(4). The incidence of rotavirus infection is registered in the year-round with indistinctive two peaks in July and August.
Databáze: Directory of Open Access Journals
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