Sulforaphane Bioavailability in Healthy Subjects Fed a Single Serving of Fresh Broccoli Microgreens

Autor: John A. Bouranis, Carmen P. Wong, Laura M. Beaver, Sandra L. Uesugi, Ethan M. Papenhausen, Jaewoo Choi, Edward W. Davis, Adilson Nunes Da Silva, Newton Kalengamaliro, Rekha Chaudhary, Jordan Kharofa, Vinita Takiar, Thomas J. Herzog, William Barrett, Emily Ho
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Foods, Vol 12, Iss 20, p 3784 (2023)
Druh dokumentu: article
ISSN: 2304-8158
DOI: 10.3390/foods12203784
Popis: Cruciferous vegetable consumption is associated with numerous health benefits attributed to the phytochemical sulforaphane (SFN) that exerts antioxidant and chemopreventive properties, among other bioactive compounds. Broccoli sprouts, rich in SFN precursor glucoraphanin (GRN), have been investigated in numerous clinical trials. Broccoli microgreens are similarly rich in GRN but have remained largely unexplored. The goal of this study was to examine SFN bioavailability and the microbiome profile in subjects fed a single serving of fresh broccoli microgreens. Eleven subjects participated in a broccoli microgreens feeding study. Broccoli microgreens GRN and SFN contents and stability were measured. Urine and stool SFN metabolite profiles and microbiome composition were examined. Broccoli microgreens had similar GRN content to values previously reported for broccoli sprouts, which was stable over time. Urine SFN metabolite profiles in broccoli microgreens-fed subjects were similar to those reported previously in broccoli sprouts-fed subjects, including the detection of SFN-nitriles. We also reported the detection of SFN metabolites in stool samples for the first time. A single serving of broccoli microgreens did not significantly alter microbiome composition. We showed in this study that broccoli microgreens are a significant source of SFN. Our work provides the foundation for future studies to establish the health benefits of broccoli microgreens consumption.
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