In vivo model to study the impact of genetic variation on clinical outcome of mastitis in uniparous dairy cows

Autor: L. Rohmeier, W. Petzl, M. Koy, T. Eickhoff, A. Hülsebusch, S. Jander, L. Macias, A. Heimes, S. Engelmann, M. Hoedemaker, H. M. Seyfert, C. Kühn, H. J. Schuberth, H. Zerbe, M. M. Meyerholz
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Veterinary Research, Vol 16, Iss 1, Pp 1-12 (2020)
Druh dokumentu: article
ISSN: 1746-6148
81809824
DOI: 10.1186/s12917-020-2251-8
Popis: Abstract Background In dairy herds, mastitis causes detrimental economic losses. Genetic selection offers a sustainable tool to select animals with reduced susceptibility towards postpartum diseases. Studying underlying mechanisms is important to assess the physiological processes that cause differences between selected haplotypes. Therefore, the objective of this study was to establish an in vivo infection model to study the impact of selecting for alternative paternal haplotypes in a particular genomic region on cattle chromosome 18 for mastitis susceptibility under defined conditions in uniparous dairy cows. Results At the start of pathogen challenge, no significant differences between the favorable (Q) and unfavorable (q) haplotypes were detected. Intramammary infection (IMI) with Staphylococcus aureus 1027 (S. aureus, n = 24, 96 h) or Escherichia coli 1303 (E. coli, n = 12, 24 h) was successfully induced in all uniparous cows. This finding was confirmed by clinical signs of mastitis and repeated recovery of the respective pathogen from milk samples of challenged quarters in each animal. After S. aureus challenge, Q-uniparous cows showed lower somatic cell counts 24 h and 36 h after challenge (P
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