Absorption/Attenuation Spectral Description of ESKAPEE Bacteria: Application to Seeder-Free Culture Monitoring, Mammalian T-Cell and Bacteria Mixture Analysis and Contamination Description

Autor:	Bruno Wacogne, Marine Belinger Podevin, Naïs Vaccari, Claudia Koubevi, Céline Codjiová, Emilie Gutierrez, Pauline Bourgeois, Lucie Davoine, Marjorie Robert-Nicoud, Alain Rouleau, Annie Frelet-Barrand
Jazyk:	angličtina
Rok vydání:	2023
Předmět:	white light spectroscopy absorption spectra ESKAPEE bacteria Lactococcus lactis co-culture monitoring mammalian T-cells Chemical technology TP1-1185
Zdroj:	Sensors, Vol 23, Iss 9, p 4325 (2023)
Druh dokumentu:	article
ISSN:	1424-8220
DOI:	10.3390/s23094325
Popis:	Despite numerous innovations, measuring bacteria concentrations on a routine basis is still time consuming and ensuring accurate measurements requires careful handling. Furthermore, it often requires sampling small volumes of bacteria suspensions which might be poorly representative of the real bacteria concentration. In this paper, we propose a spectroscopy measurement method based on a description of the absorption/attenuation spectra of ESKAPEE bacteria. Concentrations were measured with accuracies less than 2%. In addition, mixing the mathematical description of the absorption/attenuation spectra of mammalian T-cells and bacteria allows for the simultaneous measurements of both species’ concentrations. This method allows real-time, sampling-free and seeder-free measurement and can be easily integrated into a closed-system environment.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/d4bb1e2c1f0c48cba8e79f763678b7b1 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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