| Autor: |
Ranran Tu, Hong Wang, Zhihao Sun, Qinwen Zou, Jiajun Wu, Duo Wu, Nan Wang, Guanghua He |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Crop Journal, Vol 12, Iss 3, Pp 938-943 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2214-5141 |
| DOI: |
10.1016/j.cj.2024.04.010 |
| Popis: |
Anther dehiscence controls optimal interaction between pollen and stigma, thereby determining the successful sexual reproduction. The regulators or mechanisms of this process remain elusive. Here, two CRISPR/Cas9 mutants of a rice exocyst subunit gene SEC3A, sec3a-1 and sec3a-2, showed anther indehiscence at anthesis and male sterility at maturity. Pollen viability and germination in the mutants were partly defective, whereas their female gametes undergone a normal development. Hybrid or self-pollinated seeds could be produced by artificial pollination, suggesting potential use of a weak sec3a mutant as a female line during hybrid breeding. SEC3A is widely expressed in various tissues, including anther walls. Further results showed an excessive IAA accumulation and no endothecium lignification in sec3a-1/2 anthers. Our findings suggest that SEC3A appears to regulate anther dehiscence by modulating auxin signaling, providing insights into regulation of anther dehiscence and function of exocyst in plants. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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