| Autor: |
JIN Ming, BA Hua-jie, ZHU Ai-hua, et al |
| Jazyk: |
čínština |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Fayixue Zazhi, Vol 34, Iss 2, Pp 157-160 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
1004-5619 |
| DOI: |
10.3969/j.issn.1004-5619.2018.02.011 |
| Popis: |
Objective To explore the effect of benzidine test and related reagents on DNA analysis of bloodstain. Methods A total of 970 bloodstain filter paper samples with 1 μL venous blood were collected, and 10 of them acted as control samples. After benzidine test and related reagent processing, DNA of 960 samples was extracted by Chelex-100 and silica bead methods and then multiplex amplified by AmpF?詛STRTM IdentifilerTM Plus PCR kits. The results of STR typing were compared between different groups. Results DNA were extracted immediately after benzidine test. Totally STR loci （3.80±1.34） were detected by silica bead method, while no STR loci were obtained by Chelex-100 method. Thirteen samples （21.7%） with whole STR typing results were obtained by drying after benzidine test, and the STR locus number （12.90±1.49） which obtained by silica bead method was much higher than by Chelex-100 method （4.70±1.96） （P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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