Determination of pseudoephedrine, paracetamole and ascorbic acid in multicomponent powder using reversed-phase chromatographic method
| Autor: | Malenović Anđelija, Jančić-Stojanović Biljana, Ivanović Darko |
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| Jazyk: | srbština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Arhiv za farmaciju, Vol 64, Iss 6, Pp 473-486 (2014) |
| Druh dokumentu: | article |
| ISSN: | 0004-1963 2217-8767 |
| DOI: | 10.5937/arhfarm1406473M |
| Popis: | For the simultaneous analysis of multicomponent mixture containing pseudoephedrine, paracetamole and ascorbic acid two RP-HPLC methods were developed. Separation were performed on Hewlett Packard 1100 system which consisted of HP 1100 binary pump, HP 1100 UV/VIS detector and HP ChemStation for the automatic data evaluation. Methods were developed, e. g. chromatographic conditiond settled, methods submited to method validation and then applied for the determination of content of multicomponent powder.The best separation was achived on Platinium Alltech Amino 150 x 4.6 mm, 5 mm particle size column, at 30°C. Injection volume was 20mL. Mobile phase in mehtod I was a mixture of methanol-water (30:70 V/V), pH 2.5, and in mehtod II was a mixture methanol-water (60:40 V/V), pH 2.9. pH of the mobile phases were adjusted with 85% ortophosphoric acid. Detection was performed at 214 nm in mehtod I and at 257 nm in method II. Flow rate was 1 mLmin-1. Developed methods were validated. Obtained results showed that both methods are suitable for routine analysis of pharmaceutical dosage forms which contain pseudoephedrine, paracetamole and ascorbic acid. |
| Databáze: | Directory of Open Access Journals |
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