Production and Characterization of Cross-Linked Aggregates of Geobacillus thermoleovorans CCR11 Thermoalkaliphilic Recombinant Lipase

Autor: Rosa-María Oliart-Ros, Giselle-Lilian Badillo-Zeferino, Rodolfo Quintana-Castro, Irving-Israel Ruíz-López, Alfonso Alexander-Aguilera, Jorge-Guillermo Domínguez-Chávez, Azmat Ali Khan, Dinh Duc Nguyen, Ashok Kumar Nadda, María-Guadalupe Sánchez-Otero
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Molecules, Vol 26, Iss 24, p 7569 (2021)
Druh dokumentu: article
ISSN: 26247569
1420-3049
DOI: 10.3390/molecules26247569
Popis: Immobilization of enzymes has many advantages for their application in biotechnological processes. In particular, the cross-linked enzyme aggregates (CLEAs) allow the production of solid biocatalysts with a high enzymatic loading and the advantage of obtaining derivatives with high stability at low cost. The purpose of this study was to produce cross-linked enzymatic aggregates (CLEAs) of LipMatCCR11, a 43 kDa recombinant solvent-tolerant thermoalkaliphilic lipase from Geobacillus thermoleovorans CCR11. LipMatCCR11-CLEAs were prepared using (NH4)2SO4 (40% w/v) as precipitant agent and glutaraldehyde (40 mM) as cross-linker, at pH 9, 20 °C. A U10(56) uniform design was used to optimize CLEA production, varying protein concentration, ammonium sulfate %, pH, glutaraldehyde concentration, temperature, and incubation time. The synthesized CLEAs were also analyzed using scanning electron microscopy (SEM) that showed individual particles of
Databáze: Directory of Open Access Journals
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