| Autor: |
Amira Ben Amara, Marie-Sarah Rouviere, Stéphane Fattori, Julia Wlosik, Emilie Gregori, Nicolas Boucherit, Pierre-Louis Bernard, Jacques A. Nunès, Norbert Vey, Herve Luche, Laurent Gorvel, Daniel Olive, Anne-Sophie Chretien |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 4, Pp 101768- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101768 |
| Popis: |
Summary: This protocol details the step-by-step procedure for in-depth immune phenotyping of peripheral blood natural killer (NK) cells from clinical samples by mass cytometry. The protocol consists of three main steps: PBMC incubation with a mix of metal-conjugated antibodies for extracellular phenotyping followed by fixation, permeabilization and incubation with a mix of metal-conjugated antibodies for staining of intracellular proteins, and sample acquisition on a mass cytometer. High-dimensional analysis enables the visualization of NK cell subsets and their phenotypical characteristics.For complete details on the use and execution of this protocol, please refer to Chretien et al. (2021). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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