The effect of chrysin–curcumin-loaded nanofibres on the wound-healing process in male rats
| Autor: | Zoheyr Mohammadi, Mohsen Sharif Zak, Hasan Majdi, Ebrahim Mostafavi, Meisam Barati, Hamid Lotfimehr, Kambiz Ghaseminasab, Hamidreza Pazoki-Toroudi, Thomas J. Webster, Abolfazl Akbarzadeh |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Artificial Cells, Nanomedicine, and Biotechnology, Vol 47, Iss 1, Pp 1642-1652 (2019) |
| Druh dokumentu: | article |
| ISSN: | 21691401 2169-141X 2169-1401 |
| DOI: | 10.1080/21691401.2019.1594855 |
| Popis: | Aim The aim of the present study was to produce chrysin–curcumin-loaded PCL-PEG nanofibres by an electrospinning technique and to evaluate the biological activity of the chrysin–curcumin-loaded PCL-PEG fibres for wound healing and its related genes using in vivo methods.Materials and methods The electrospinning method was carried out for the preparation of the chrysin, curcumin and chrysin–curcumin-loaded PCL-PEG nanofibres with different concentrations. FTIR and SEM were performed to characterize the chemical structures and morphology of the nanofibres. In vitro drug release, as well as in vivo wound-healing studies were investigated in male rats. The expressions of genes related to the wound-healing process were also evaluated by real-time PCR.Results Our study showed that the chrysin–curcumin-loaded nanofibres have anti-inflammatory properties in several stages of the wound-healing process by affecting the IL-6, MMP-2, TIMP-1, TIMP-2 and iNOS gene expression. Our results demonstrated that the effect of the chrysin-loaded nanofibre, the curcumin-loaded nanofibre and the chrysin–curcumin-loaded nanofibre in the wound-healing process is dose dependent and in accordance with the obtained results in that it might affect the inflammation phase more than the other stages of the wound-healing process.Conclusion We have introduced chrysin–curcumin-loaded PCL-PEG nanofibres as a novel compound for shortening the duration of the wound-healing process. |
| Databáze: | Directory of Open Access Journals |
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