Morphological aspects and distribution of granules composed of deproteinized bovine bone or human dentin into a putty mixture: an in vitro study

Autor: Inês Pimentel, Bruno Henriques, Filipe Silva, Oscar Carvalho, Wim Teughels, Mutlu Özcan, Júlio C. M. Souza
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Head & Face Medicine, Vol 19, Iss 1, Pp 1-12 (2023)
Druh dokumentu: article
ISSN: 1746-160X
DOI: 10.1186/s13005-023-00398-7
Popis: Abstract Objective The main aim of this study was to evaluate the morphological aspects and distribution of granules composed of deproteinized bovine bone mineral (DBBM) and human dentin-derived bone graft (HDBG) into a putty consistency mixture. Materials and methods DBBM or HDBG were mixed with an alginate-based hydrogel at two different granule/hydrogel ratio (1:1 and 1:3) and divided into four test groups while two control groups were composed of DBBM or HDBG free of hydrogel. Groups of specimens were cross-sectioned for morphological evaluation by scanning electron microscopy (SEM) at backscattered electrons mode. Details on the dimensions and pores’ size of DBBM and HDBG were evaluated after mixing different amounts of particles and alginate-based hydrogels. Results Microscopic analyses revealed a size of DBBM granules ranging from 750 up to 1600 μm while HDBG particles showed particle size ranging from 375 up to 1500 μm. No statistical differences were identified regarding the size of granules (p > 0.5). The mean values of pores’ size of DBBM particles were noticed at around 400 μm while HDBG particles revealed micro-scale pores of around 1–3 μm promoted by the dentin tubules (p
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