The role of miR-369-3p in proliferation and differentiation of preadipocytes in Aohan fine-wool sheep

Autor: S. Xue, K. Liu, L. Zhao, L. Zhou, X. Gao, L. Liu, N. Liu, J. He
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Archives Animal Breeding, Vol 66, Pp 93-102 (2023)
Druh dokumentu: article
ISSN: 0003-9438
2363-9822
DOI: 10.5194/aab-66-93-2023
Popis: MicroRNAs (miRNAs) are a large class of non-coding RNAs that play important roles in the proliferation and differentiation of adipocytes. Our previous sequencing analysis revealed higher expression of miR-369-3p in the longissimus muscle of 2-month-old Aohan fine-wool sheep (AFWS) compared to 12-month-old sheep (P), suggesting that miR-369-3p may regulate fat deposition in AFWS. To test this, miR-369-3p mimics, inhibitors, and negative controls (NCs) were constructed and transfected into AFWS preadipocytes. After transfection with miR-369-3p mimics, we found a decrease (P) in the expression of genes and proteins related to cell proliferation and differentiation, detected by RT-qPCR (quantitative reverse transcription PCR) and western blot analyses. Moreover, EdU (5-ethynyl-2′-deoxyuridine) detection and Oil Red O staining showed a decrease (P) in cell proliferation and lipid accumulation, respectively. The opposite trends (P) were obtained after transfection with miR-369-3p inhibitors. In conclusion, the results showed that miR-369-3p can inhibit the proliferation and differentiation of AFWS preadipocytes, providing a theoretical basis to further explore the molecular mechanism of fat deposition in sheep and other domestic animals.
Databáze: Directory of Open Access Journals
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