Dynamic alteration and prognostic significance of tumor‐associated CD68+ and CD68+PD‐L1− macrophages in muscle‐invasive bladder cancer treated with neoadjuvant chemotherapy

Autor: Jie Wu, Rui‐Yang Xie, Li‐Hui Wei, Chuan‐Zhen Cao, Bing‐Qing Shang, You‐Yan Guan, Hong‐Zhe Shi, Wang Qu, Yun Li, Jing Liang, Shan Zheng, Ai‐Ping Zhou, Xiao‐Feng Zhou, Jian‐Zhong Shou, Xin‐Gang Bi
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Cancer Medicine, Vol 12, Iss 4, Pp 4981-4992 (2023)
Druh dokumentu: article
ISSN: 2045-7634
DOI: 10.1002/cam4.5191
Popis: Abstract Background The current study aimed to investigate the dynamic alteration and prognostic significance of tumor‐infiltrating lymphocytes (TILs), tumor‐associated macrophages (TAMs), and PD‐L1 status of immune cells in muscle‐invasive bladder cancer (MIBC) treated with neoadjuvant chemotherapy (NAC). Methods Multiplex immunofluorescence staining was performed to examine CD68+ TAM, CD4+ T cell, CD8+ T cell, FOXP3+ Treg cell, and PD‐L1 expression in paired MIBC tissues (n = 54) before and after NAC. Patients were then divided into definite responders (DR), (≤pT1) and incomplete responders (IR). Results There was no significant difference between DR and IR cohorts for the immune cell infiltration levels at the baseline status. Tobacco history was identified to be associated with worse NAC efficacy. CD68+ (stroma area: p = 0.025; tumor area: p = 0.028; total area: p = 0.013) and CD68+PD‐L1− (stroma area: p = 0.035; tumor area: p = 0.013 total area: p = 0.014) TAMs infiltration levels decreased significantly after NAC, while there was no significant difference of CD68+PD‐L1+ and TILs. The infiltration of CD68+ (p = 0.033), CD68+PD‐L1− (p = 0.033), and CD68+PD‐L1+ (p
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