Hypoxia Upregulates the Expression of Annexin A1 in Lung Adenocarcinoma A549 Cells

Autor: Zhenhong HU, Jian HUANG, Zhenhua LI, Xiaoxu LI, Zuhong FU, Haichao LIU, Libin GUAN
Jazyk: čínština
Rok vydání: 2012
Předmět:
Zdroj: Chinese Journal of Lung Cancer, Vol 15, Iss 5, Pp 277-280 (2012)
Druh dokumentu: article
ISSN: 1009-3419
1999-6187
DOI: 10.3779/j.issn.1009-3419.2012.05.05
Popis: Background and objective The growth of tumor often faced up with lackness of blood and oxygen, and it has been reported that Annexin A1 may be involved in tumor. The aim of this investigation is to explore the characteristics of expression of Annexin A1 in lung adenocarcinoma A549 cells after hypoxia. Methods A549 cells were exposured to either normoxia (21%O2) or hypoxia (1%O2) condition for 4 h, 12 h, 24 h. The expressions of Annexin A1 mRNA levels were measured by RT-PCR. The expressions of Annexin 1 protein were investigaged by Western blot. The relative content of reactive oxygen species (ROS) were assayed by special kit. The expressions of nuclear translocation of NF-κB was assayed by Western blot; After been treated with ROS scavenger NAC and PDTC, the levels of Annexin 1 protein of A549 cells were measured by Western blot. Results Compared with normoxia group, the Annexin A1 mRNA in hypoxia group increased after 4 h, and then decreased gradually; Moreover, Annexin 1 protein levels of A549 cells were also increased when treated with hypoxia. An increaing of ROS production in cells exprosed to hypoxia was detected. NAC and PDTC inhibited hypoxia-induced Annexin A1 increase. Conclusion Hypoxia upregulates the expression of Annexin A1 in lung adenocarcinoma A549 cells, in which process ROS-NF-κB may paticipate in.
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