Improvement of image resolution by combining enhanced confocal microscopy and quantum dot triexciton imaging

Autor: Simon Hennig, Dietmar J. Manstein
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: FEBS Open Bio, Vol 11, Iss 12, Pp 3324-3330 (2021)
Druh dokumentu: article
ISSN: 2211-5463
DOI: 10.1002/2211-5463.13246
Popis: Super‐resolution fluorescence imaging provides critically improved information about the composition, organization, and dynamics of subcellular structures. Quantum dot triexciton imaging (QDTI) has been introduced as an easy‐to‐use sub‐diffraction imaging method that achieves an almost 2‐fold improvement in resolution when used with conventional confocal microscopes. Here, we report an overall 3‐fold increase in lateral and axial resolution compared to conventional confocal microscopes by combining QDTI with state‐of‐the‐art commercial laser scanning microscope systems.
Databáze: Directory of Open Access Journals