Inhibition of miR-19a protects neurons against ischemic stroke through modulating glucose metabolism and neuronal apoptosis

Autor: Xiao-Li Ge, Jin-Li Wang, Xin Liu, Jia Zhang, Chang Liu, Li Guo
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Cellular & Molecular Biology Letters, Vol 24, Iss 1, Pp 1-11 (2019)
Druh dokumentu: article
ISSN: 1425-8153
1689-1392
DOI: 10.1186/s11658-019-0160-2
Popis: Abstract Background Accumulating evidence has shown that altered microRNA (miR) modulation is implicated in the pathologies of ischemic stroke. However, it is unclear whether and how hsa-miR-19a-3p mediates cerebral ischemic injury. Herein, we investigated the functional role of miR-19a-3p in cerebral ischemic injury and explored its underlying regulatory mechanism. Methods In vivo ischemic/reperfusion (I/R) neuronal injury and in vitro oxygen-glucose deprivation (OGD) were established. Expression of miR-19a-3p was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Glucose uptake, lactate production, and apoptosis were determined. ADIPOR2 was predicted as a target of miR-19a-3p in silico and experimentally validated by qRT-PCR, Western blot analysis and luciferase assay assays. Results MiR-19a expression was significantly downregulated and upregulated in rat neurons and astrocytes, respectively (P
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