Increased Serpina3n release into circulation during glucocorticoid‐mediated muscle atrophy

Autor: Marine Gueugneau, Donatienne d'Hose, Caroline Barbé, Marie deBarsy, Pascale Lause, Dominique Maiter, Laure B. Bindels, Nathalie M. Delzenne, Laurent Schaeffer, Yann‐Gaël Gangloff, Christophe Chambon, Cécile Coudy‐Gandilhon, Daniel Béchet, Jean‐Paul Thissen
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Cachexia, Sarcopenia and Muscle, Vol 9, Iss 5, Pp 929-946 (2018)
Druh dokumentu: article
ISSN: 2190-6009
2190-5991
DOI: 10.1002/jcsm.12315
Popis: Abstract Background Glucocorticoids (GC) play a major role in muscle atrophy. As skeletal muscle is a secretory organ, characterization of the muscle secretome elicited by muscle atrophy should allow to better understand the cellular mechanisms and to identify circulating biomarkers of this condition. Our project aimed to identify the changes in the muscle secretome associated with GC‐induced muscle atrophy and susceptible to translate into circulation. Methods We have identified the GC‐induced changes in the secretome of C2C12 muscle cells by proteomic analysis, and then, we have determined how these changes translate into the circulation of mice or human subjects exposed to high concentrations of GC. Results This approach led us to identify Serpina3n as one of the most markedly secreted protein in response to GC. Our original in vitro results were confirmed in vivo by an increased expression of Serpina3n in skeletal muscle (3.9‐fold; P
Databáze: Directory of Open Access Journals
Externí odkaz: