Determination of concentration 2,4-D and 6-BAP to induction morphogenic callus of Sweet Potato
| Autor: | Orlando S. González Paneque, Isabel Milanés Vega, Juan J. Silva Pupo, Angel Espinosa Reyes, Leonardo Acosta Pompa |
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| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2003 |
| Předmět: | |
| Zdroj: | Biotecnología Vegetal, Vol 3, Iss 1 (2003) |
| Druh dokumentu: | article |
| ISSN: | 1609-1841 2074-8647 |
| Popis: | Tubers from Inivit B 90-1 and Yabú-8, cultivars were put in the laboratories into water bottle and we chose explants of limbs from sprout. Explants were disinfected with Sodium Hypoclorite (Na OCl) with active chlorine (1%) and they were put into Murashige and Skoog culture medium under different concentrations of 2,4-D independent and combined with 6-BAP in the culture medium. Crops were put on the energy illumination. The best results in four weeks at the morfogenic callus induction were obtained with the concentrations of 2,4-D (0.1 mg.l-1) and 2,4-D (0.1 mg.l-1) combined with BAP (0.2 mg.l-1) in the culture medium. Key words: clon, explants, Ipomoea, tubers |
| Databáze: | Directory of Open Access Journals |
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