| Autor: |
T.A. Beacham, V. Mora Macia, P. Rooks, D.A. White, S.T. Ali |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Biotechnology Reports, Vol 7, Iss C, Pp 87-94 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2215-017X |
| DOI: |
10.1016/j.btre.2015.05.007 |
| Popis: |
Microalgae have potential as a chemical feed stock in a range of industrial applications. Nannochloropsis salina was subject to EMS mutagenesis and the highest lipid containing cells selected using fluorescence-activated cell sorting. Assessment of growth, lipid content and fatty acid composition identified mutant strains displaying a range of altered traits including changes in the PUFA content and a total FAME increase of up to 156% that of the wild type strain. Combined with a reduction in growth this demonstrated a productivity increase of up to 76%. Following UV mutagenesis, lipid accumulation of the mutant cultures was elevated to more than 3 fold that of the wild type strain, however reduced growth rates resulted in a reduction in overall productivity. Changes observed are indicative of alterations to the regulation of the omega 6 Kennedy pathway. The importance of these variations in physiology for industrial applications such as biofuel production is discussed. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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