| Autor: |
Silviya Abarova, Katerina Stoitchkova, Svetlin Tzonev, Maria Argirova, Denitsa Yancheva, Neda Anastassova, Boris Tenchov |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Pharmacia, Vol 71, Iss , Pp 1-5 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2603-557X |
| DOI: |
10.3897/pharmacia.71.e112385 |
| Popis: |
In the present work we studied the interactions of a newly synthesized drug candidate, 2-(2-hydroxy-4-methoxybenzylidene)-1-(1H-benzimidazol-2-yl)hydrazine (2H4MBBH), with human serum albumin (HSA) by fluorescence spectroscopy. 2H4MBBH-HSA binding parameters were assessed by fluorescence quenching strategy. As made clear by the concentration data, 2H4MBBH unequivocally quenched the instrinsic HSA fluorescence. The calculated Stern-Volmer quenching constant Ksv, the Ka of 2H4MBBH-HSA complexes, as well as the thermodynamic parameters ∆H°, ∆S° and ∆G°, showed that the H-bonding forces play major part in the interaction of 2H4MBBH with HSA. These calculations point to a quenching component based on 2H4MBBH-HSA static complex formation rather than energetic collisions. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
